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Role of F-actin-binding by the influenza virus nucleoprotein

ReferenceBBS/E/I/00000725
Principal Investigator / Supervisor Dr John McCauley
Co-Investigators /
Co-Supervisors
Institution The Pirbright Institute
DepartmentThe Pirbright Institute Department
Funding typeResearch
Value (£) 14,230
StatusCompleted
TypeInstitute Project
Start date 01/10/1998
End date 30/09/2001
Duration36 months

Abstract

This project is designed to investigate the interaction between influenza virus ribonucleoprotein complexes with elements of the cellular cytoskeleton and the plasma membrane of infected cells. The role of actin in the production of the filamentous form of influenza viruses will be examined through the use of drugs that affect actin polymerization and high-resolution atomic force microscopy will be used to investigate the nature of the budding of filamentous virus. Confocal microscopy will also be used to characterize the steps in virion assembly. Analysis of lipid rafts will also be done to determine their role in budding virus. Objectives: 1. To determine the role of actin in the production of the filamentous form of influenza viruses. 2. To determine the interaction between lipid-raft domains and cellular and virus components during virus assembly.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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