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Generation of recombinant viruses to study assembly of African swine fever virus (ASFV)

Reference	BBS/E/I/00000723
Principal Investigator / Supervisor	Professor Tom Wileman
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	50,530
Status	Completed
Type	Institute Project
Start date	01/10/1998
End date	30/09/2001
Duration	36 months

Abstract

We are using African Swine Fever virus as a model system to study the assembly and envelopment of large DNA viruses. Most viruses gain a single membrane envelope by budding into intracellular membrane compartments. For large DNA viruses, however, this process is more complex and large virions are wrapped by membrane cisternae derived from specific cellular membrane compartments. Wrapping provides two membrane envelopes in one step, and membrane glycoproteins become buried in the interior of the virion. This project will exploit the recent sequencing of the entire 150kbp genome of ASF virus to identify membrane proteins and proteins with membrane targeting sequences that may direct envelopment of ASF virus. We will use cryo- electron microscopy and immunogold techniques to study the distribution of these proteins between host and virus membranes, and develop cell biological assays to probe the biochemical mechanisms that control the recruitment of membrane cisternae into virus assembly sites.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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