Award details

Replication of infectivity in vitro

Reference	BBS/E/I/00000657
Principal Investigator / Supervisor	Dr James Hope
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	116,407
Status	Completed
Type	Institute Project
Start date	02/10/1997
End date	01/10/2000
Duration	36 months

Abstract

On binding to PrPSc in vitro, PrPC becomes more resistant to hydrolysis, epitopes in its 90-120 region are masked and the altered 'PrPC' can be pelleted from solution in association with PrPSc. By these biophysical, biochemical and immunological criteria several which have long been used to define the infectious prion several the PrPC has been converted to PrPSc. If PrPSc is infectious then it should be possible to show the de novo production of infectivity by bioassay. Current failure to show 'new' infectious particles may reflect technical inadequacy or the inadequacy of the prion hypothesis itself. Novel methods of high- efficiency conversion and protein fractionation are in development to underpin future biological assay investigations of this idea.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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