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Award details
Development of an ultra-sensitive, time-resolved fluoro- immunoassay for PrPSc
Reference
BBS/E/I/00000627
Principal Investigator / Supervisor
Dr James Hope
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
10,760
Status
Completed
Type
Institute Project
Start date
01/04/1997
End date
31/12/1998
Duration
21 months
Abstract
Cellular prion protein (PrPC) is normally found attached to plasma membranes by a C-terminal glycolipid anchor. This glycoprotein is the precursor of PrPSc, a disease-specific isoform, which accumulates in brain and other tissues during the pathogenesis of scrapie, BSE and other transmissible spongiform encephalopathies. PrPSc differs in shape from PrPC : it is less soluble, more aggregated and more protease-resistant than its normal isoform. Some monoclonal antibody (mcAb) epitopes on the surface of PrPC are masked in PrPSc and the mcAb's to the codon 90-120 region of the molecule (such as 3F4) can only recognise PrPSc efficiently after it has been disaggregated and denatured. The IAH have developed an ultra-sensitive, time-resolved fluoroimmunoassay for PrP using two mcAb's, FH11 and 3F4. This assay is being used to measure PrP in brain extracts and body fluids.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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