Award details

Differential control of PrP gene expression in sheep brain and periphery

Reference	BBS/E/I/00000622
Principal Investigator / Supervisor	Professor Nora Hunter
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	64,234
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1999
Duration	30 months

Abstract

The aim of this project is to confirm the importance of the controlling element and to change to using sheep cell lines in order to study sheep PrP mRNA in sheep cell lines. This project will establish whether both mRNAs are actively expressed in sheep cells and whether there are any tissue specific differences or association with scrapie susceptibility. The processing and cellular location of PrP mRNA and protein encoded by the two different mRNAs will be studied in transient expression experiments in sheep cell lines (brain and peripheral tissue origin) of defined PrP genotype and therefore susceptibility to scrapie. Such experiments have not previously been carried out in cell lines from this, the natural host of scrapie. The 4.6kb and the 2.1kb mRNA species will be studied separately using specific cloned DNA constructs. Comparisons will be made between cell lines from scrapie susceptible and resistant sheep.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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