Award details

Characterisation and cloning of avian cytokines

Reference	BBS/E/I/00000452
Principal Investigator / Supervisor	Professor Peter Kaiser
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	177,522
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/2000
Duration	36 months

Abstract

We have carried out genomic studies (cloning and sequencing the gene and its promoter, identifying promoter polymorphisms between inbred lines of chickens, and mapping the gene) on several chicken cytokine genes including interferon- (IFN- ), interleukin-2 (IL-2) and IL-8, in the process clarifying the identity of the latter two molecules. Both IL-2 and IL-8 show synteny of genetic location between chickens and mammals. We have expressed bioactive recombinant chicken IFN- and IL-2, and are raising monoclonal antibodies to them. We also cloned the genes for IFN- from several species of galliforms. The high degree of overall identity (87.8-97.6%) at the predicted primary amino acid sequence level of the protein, including the deduced IFN- receptor binding motifs, suggests that IFN- may be cross-reactive among these species.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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