Award details

Trafficking and binding interactions of microneme proteins of Eimeria tenella

Reference	BBS/E/I/00000429
Principal Investigator / Supervisor	Professor Fiona Tomley
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	247,246
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/2000
Duration	36 months

Abstract

The objectives of this project are to understand the mechanisms by which microneme secretion is induced in Eimeria sporozoites and to identify host and parasite molecules involved. Soluble components of host cells that bind to sporozoites and induce microneme secretion will be identified by HPLC fractionation of tissue culture supernatants and the corresponding sporozoite binding partners will be identified by affinity binding and far- western blotting. Specific antisera will used to localise these protein(s) in the sporozoite, for molecular cloning and to assess whether the antisera can block sporozoite invasion into MDBK cells. The roles of calcium signalling, protein phosphorylation, temperature and the parasite cytoskeleton in microneme secretion and host cell invasion will also be investigated using inhibitors.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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