Award details

Structure and organisation of the microneme organelles of Eimeria tenella

Reference	BBS/E/I/00000428
Principal Investigator / Supervisor	Professor Fiona Tomley
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	302,562
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/2000
Duration	36 months

Abstract

Microneme organelles are found in all apicomplexan parasites and contain proteins that are critical for parasite motility and adhesion to host cells. The objectives of this project are to clone proteins from the micronemes and to initiate studies on how each of these proteins is involved in host cell invasion. Subcellular fractionation will be used to isolate micronemes and to purify individual microneme proteins. Specific antisera will be used for molecular cloning, to confirm the microneme localisation of individual molecules and to examine their proteolytic processing, membrane attachment and secretion during host cell invasion. Specific antisera and over- expressed, purified recombinant microneme proteins will be used to investigate which microneme proteins bind directly to host cells and to determine whether antibodies or proteins can inhibit the invasion of sporozoites into MDBK cells.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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