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CD4 recognition of respiratory syncytial virus proteins

Reference	BBS/E/I/00000394
Principal Investigator / Supervisor	Dr Geraldine Taylor
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	129,588
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1999
Duration	30 months

Abstract

The two surface glycoproteins of respiratory syncytial virus (RSV) are the major protective antigens of the virus. In order to determine how recognition of these two proteins by bovine CD4+ T cells is influenced by bovine MHC class II, overlapping synthetic peptides were used to map T-cell epitopes. Analysis of proliferative responses of bovine peripheral blood mononuclear cells (PBMC) from MHC class II- typed calves to the synthetic peptides, showed that calves recognised epitopes predominantly in the F1 subunit of the F protein. A number of calves with different class II haplotypes recognised peptides in the region 291-321 of the F protein and some animals recognised epitopes adjacent to or overlapping with previously defined B cell epitopes. Whereas several epitopes were identified in the F protein, only one dominant epitope was identified in the G protein. This epitope, in the cytoplasmic/transmembrane region of the G protein, was recognised by PBMC from at least 5 calves with different class II haplotypes, and there was little recognition of the extracellular domain of the G protein. It may be that the extensive glycosylation of the extracellular domain of the G protein inhibits processing of this part of the protein

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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