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Identification & characterisation of genes involved in signal reception in growth down-regulation in stationary phase growth in Salmonella typhimurium

Reference	BBS/E/I/00000360
Principal Investigator / Supervisor	Professor Paul Barrow
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	39,777
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1999
Duration	30 months

Abstract

The characteristics of growth of food-poisoning Salmonella strains in the alimentary tract are similar to those in stationary-phase nutrient broth cultures. We are trying to identify those microbial characteristics which the bacterial cell uses to recognize that it is in stationary-phase. Initial studies centered on the use of in vitro screening procedures to allow the identification of mutants of S. typhimurium which are able to multiply in a 24h broth culture of the parent strain. A single mutant was identified which showed the characteristic. DNA sequencing showed that the transposon insertion was in a previously unidentified open reading frame. Further studies are concentrating on the interaction between the proton translocating apparatus, used in respiration, and which we have previously shown to be involved in this phenomenon, and regulation of cell division and other stationary- phase functions. This is being done by constructing lux fusions in rpoS (stationary-phase sigma factor) and sdiA (regulator of cell division) and assessing the affect of mutations in the electron transport genes nuo, cyd and unc.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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