Award details

Bluetongue virus: cell attachment and entry mechanism

Reference	BBS/E/I/00000250
Principal Investigator / Supervisor	Dr Andrew Metaxas
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	44,391
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1999
Duration	30 months

Abstract

The project seeks to identify the proteins, sites and mechanisms involved in initiation of BTV infection. BTV core particles have entirely lost their outer capsid proteins and therefore infect cells by a mechanism that is dependent on the outer core protein VP7(T13). Cores are highly infectious for insect vector cells and may play a role in vector status of the insect. The ability of MAbs's specific for VP7(T13) to neutralise core is being be examined. The binding of BTV glycosaminoglycans (GAGs) on the surface of insect vector (KC) and mammalian (BHK) cells and the effect of GAG modifying enzymes is being studied. Virus particle entry is also being studied using lysosomotropic agents. A recombinant CHO cell line expressing the murine Fc-RII receptor is being used to analyse and role of the BTV core particle receptor" in facilitating cell entry via receptor mediated endocytosis.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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