Award details

Cloning chicken cytokine gene clusters

Reference	BBS/E/I/00000241
Principal Investigator / Supervisor	Professor Peter Kaiser
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	54,590
Status	Completed
Type	Institute Project
Start date	01/10/1998
End date	30/09/2001
Duration	36 months

Abstract

Progress in identifying cytokine genes in chickens has been slow because of limited DNA sequence homologies with mammals, lack of specific bioassays, lack of cross- reactivity, etc. The rapid systematic identification of these molecules would make a significant contribution to the development of chicken immunology in a wide context. In mammals, cytokine genes are often clustered on particular chromosomes. The immediate aims of this project are to map chicken genes known to be associated with these cytokine gene clusters in mammals. There are several examples of synteny between human, mouse and chicken chromosomes in terms of gene order/location, including the chromosomal location of IL-2 in all three species. The mapping information and the genes will then be used to clone YACs containing these genes and genomic sequences adjacent to them. An exon trapping system will then be utilised to generate a cDNA library containing representatives of all the genes encoded on the YACs. These will be sequenced to identify them where possible by sequence homology. Full length cytokine cDNAs will then be isolated.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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