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Award details
Development of methods for the identification and selection of mapped genes, targeting a recently mapped resistance gene in chickens
Reference
BBS/E/I/00000239
Principal Investigator / Supervisor
Dr N Bumstead
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
306,052
Status
Completed
Type
Institute Project
Start date
01/04/1998
End date
30/04/2001
Duration
37 months
Abstract
This project will investigate and develop methods for the identification and selection of mapped genes, focusing on a novel salmonellosis-resistance gene which we have recently mapped to chicken chromosome 5. Additional markers in the region of this gene will be generated through construction of a chromosome 5 flow-sorted library and through Representational Difference Analysis: these markers will be used to precisely locate the resistance gene and to isolate YAC clones containing the gene. Genes within these YACs will be identified by exon trapping, ORF trapping and sequence skimming, and the identity of the gene confirmed by its pattern of expression and sequence comparisons. At the same time methods for selection for resistant animals in commercial flocks using gel-free systems will be investigated in collaboration with Ross Breeders UK Ltd.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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