Award details

Identification, by positional mapping, of genes influencing disease resistance

Reference	BBS/E/I/00000237
Principal Investigator / Supervisor	Dr N Bumstead
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	221,544
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/2000
Duration	36 months

Abstract

1. Refine the position of the resistance genes SAL1 and MDV1. 2. Investigate the properties of SAL1 and MDV1 in other lines and crosses, including commercial flocks. 3. Begin the process of isolation of SAL1 and MDV1 by identification of AC clones from the closest genetic markers. In previous work we have identified a resistance gene with strong effects on resistance to salmonellosis, SAL1, and also several genes affecting resistance to Marek's disease. In particular we have identified a gene on chromosome 1 (MDV1) which significantly affects resistance to Marek's disease by reducing the proliferation of the virus in the lymphocytes of infected birds. The gene affecting resistance to salmonellosis appears to lie on a microchromosome. At present these genes have been identified through genomic mapping using linked markers, and their functions and molecular identify are not known. We can use the existing markers to assess the effects of these genes in birds of different genetic backgrounds and in commercial flocks, and this may also provide indications of their mode of actin, which will be of assistance in identifying the genes. However, to identify the genes at the molecular level, and to select for these genes effectively in commercial circumstances, it will be necessary to generate new markers in the relevant regions which are closer to the resistance genes. We then aim to identify the resistance genes within yeast artificial chromosome (YAC) contigs constructed across the regions containing the resistance genes.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search