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Assembly of nucleoprotein core of African swine fever virus

Reference	BBS/E/I/00000224
Principal Investigator / Supervisor	Professor Tom Wileman
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	58,449
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1999
Duration	30 months

Abstract

African Swine Fever (ASF) virus is a large icosahedral enveloped DNA virus that causes a fatal haemorrhagic disease of domestic pigs. The virus is 200nm in diameter with a central nucleoprotein core surrounded by protein matrix, lipid envelopes and capsid. We are interested in the mechanism of assembly of the nucleoprotein core and subsequent insertion of the 170kbp genome into virus particles. Electron microscopy studies using DNA specific antibodies and virus specific DNA probes suggest that the genome is inserted at a late stage of morphogenesis. Biochemical experiments argue against synthesis of a pre- formed nucleoprotein core and suggest that nucleoprotein core assembly and genome packaging are co-ordinated with capsid assembly and envelopment by the endoplasmic reticulum membrane.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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