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Award details
Effect of mutations, specific to very virulent infectious bursal disease virus, on functions involved in virus replication
Reference
BBS/E/I/00000182
Principal Investigator / Supervisor
Professor Michael Skinner
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
37,384
Status
Completed
Type
Institute Project
Start date
01/04/1997
End date
30/09/1998
Duration
18 months
Abstract
This project is part of a programme on the molecular determinants of enhanced virulence of 'very virulent' (VV) infectious bursal disease virus (IBDV). In 120/050071, the complete coding sequences of both segments of a representative UK VV strain (UK661) were determined. Derived amino acid sequences revealed more divergence of UK661, from earlier strains, in the (presumed) viral protease (VP4), the viral polymerase (VP1) and structural protein VP3 than in structural protein VP2 (even though VP2, which carries the major, immunodominant, conformational, neutralising epitope, was the most variable protein amongst earlier strains. In the absence of a reverse genetics system for IBDV, the aims of the project are to investigate the potential of specific mutations to account for the enhanced virulence of VV IBDV, concentrating on mutations, especially those in RdRp motif I, that might affect the rate of viral replication by altering the activity of the VP1 RNA- dependent RNA polymerase (RdRp). The project will require the establishment of biochemical assays for VP1 functions (including: covalent and non-covalent RNA binding, priming, transcription and replication).
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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