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Mechanism of internal ribosome entry site complementation in foot and mouth disease virus

Reference	BBS/E/I/00000055
Principal Investigator / Supervisor	Professor Graham Belsham
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	29,445
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/11/1997
Duration	8 months

Abstract

Initiation of picornavirus (eg foot-and-mouth disease virus, FMDV) protein synthesis is directed by a region of about 450 bases within the 5' non-coding region termed the internal ribosome entry site (IRES). Defective IRES elements can be readily obtained by modification of their sequence. These elements are assayed within the context of bicistronic mRNAs with the IRES as the spacer elements between two reporter gene sequences. Surprisingly co- expression of a wild type IRES, unlinked to any coding sequences, in the same cells as the defective element leads to enhanced translation of the downstream reporter gene. This process is referred to as complementation. It has been shown to occur with three different genera of picornavirus IRES and is efficient, sequence specific and independent of recombination events. Both point mutants and deletion mutants can be complemented.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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