Award details

Structure and function of the fowlpox virus homologue of mammalian plasma cell protein PC-1

Reference	BBS/E/I/00000017
Principal Investigator / Supervisor	Professor Michael Skinner
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	9,878
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1997
Duration	6 months

Abstract

Sequence determination of part of the genome of fowlpox virus revealed the presence of an open reading frame encoding a homologue of the mammalian plasma cell glycoprotein, PC-1, not found in any other viruses. PC-1 is known to have alkaline phosphodiesterase, pyrophosphatase but its biological function is unknown. The project will involve: (i) targeted deletion of FPV PC-1 to determine whether or not it is an essential function (if it proves to be non-essential the mutant virus will be examined to identify any phenotypic changes); (ii) analysis of transcription of FPV PC-1 RNA; (iii) expression of mammalian and FPV PC-1 in bacterial and poxviral expression vectors to allow: production of antisera (polyclonal and monoclonal) and biochemical analysis and (iv) use of anti-PC-1 sera to characterise native PC-1 and to localise the FPV protein within the infected cell. This project forms part of a larger programme to understand how interactions between fowlpox virus (FPV) and host cells at the molecular level influence virus host range and survival. FPV genome sequence determination revealed the an open reading frame encoding a homologue of the mammalian plasma cell glycoprotein, PC-1, not found in any other viruses (including the completely sequenced genomes of vaccinia and smallpox viruses).

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search