Award details

603: Integrated project: Arabidopsis Systems Biology

Reference	BBS/E/H/00VB0267
Principal Investigator / Supervisor	Professor Vicky Buchanan-Wollaston
Co-Investigators / Co-Supervisors
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	1,709,156
Status	Completed
Type	Institute Project
Start date	01/04/2006
End date	31/03/2007
Duration	12 months

Abstract

The overall aim of this project is to develop a systems approach to the study of three aspects of Arabidopsis biology, pathogen interactions (Peronospora and Botrytis), flowering and senescence. A user group approach generates advanced genomic resources and standardised datasets. This allows the parallel study of key regulatory networks operating simultaneously in the same biological system. We expect this approach to reveal new regulatory genes and insights into how these networks cross-talk and interact. The key resources and expertise will be developed to support and integrate the research sub-projects. Genetic resources includes a high throughput plant transformation pipeline, a coordinated cloning and vector construction pipeline to feed the plant transformation, yeast 2 hybrid and yeast 1 hybrid techniques. Amplification of 20K Arabidopsis promoters to generate a promoter chip for ChIP analysis. Large microarray datasets are being generated using CATMA slides. Highly replicated time course experiments with many time points for leaf development in long days, in short days, following transfer from short to long, following infection with Botrytis and Peronospora, will be used to generate models for gene expression. These will pinpoint key regulatory genes common or unique to the different treatments.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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