Award details

512: Exploitation of the Colletotrichum-Arabidopsis model for novel applications

Reference	BBS/E/H/00SS0231
Principal Investigator / Supervisor	Professor S Sreenivasaprasad
Co-Investigators / Co-Supervisors
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	145,069
Status	Completed
Type	Institute Project
Start date	01/04/2005
End date	31/03/2006
Duration	12 months

Abstract

Phytopathogenic fungi use varied strategies for invading the host and deriving nutrients. The novel Colletotrichum- Arabidopsis pathosystem is an excellent model for developing the resources and technologies for functional genomic analysis of hemibiotrophy - initial biotrophic phase followed by a switch to necrotrophy, and would lay the basis for gaining novel insights into our understanding of the plant-fungal interactions. The major objective of the proposed work is to develop the resources for functional and comparative genomic analysis of fungal hemibiotrophy exploiting the Colletotrichum- Arabidopsis model. This will also enable us to test this system as a heterologous expression vector for avirulence/non-host resistance determinants. Moreover, the knowledge and tools generated with this model system would impact the global Colletotrichum community as a number of pathogens of this genus are hemibiotrophs on a wide range of economically important crops including cereals and legumes and genome sequencing of C. graminicola, the maize anthracnose pathogen has recently begun. A collection of cDNA libraries enriched for host and pathogen genes preferentially expressed during hemibiotrophic infection will be established. A series of analysis based on hybridisation, sequencing and bioinformatics of the cDNAs from defined stages of the hemibiotrophic infection process will be carried out for putative functional annotation of the genes encoded. Genotypic and phenotypic characterisation of more than 30 isolates of Colletotrichum spp. originating from crucifer, legume and solanaceous hosts will be carried out to establish a set of isolates for investigating host-specificity and defence. These resources are likely to facilitate the community¿s efforts towards functional and comparative genomic analysis of hemibiotrophic host interactions in Colletotrichum.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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