Award details

505: Biosensors for plant hormones

Reference	BBS/E/H/00RN0224
Principal Investigator / Supervisor	Professor Richard Napier
Co-Investigators / Co-Supervisors
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	411,423
Status	Completed
Type	Institute Project
Start date	01/04/2005
End date	31/03/2006
Duration	12 months

Abstract

The project is directed at tissue-level, real-time biosensors for plant hormones ABA and auxin. Biosensors can be divided into selective reporter systems (biochemistry) and signal processing hardware (electronics). The work in this project will be directed at identification and presentation of proteins suitable as selective reporters. The HRI group has established an anti-ABA antibody fragment (scFv) as a durable and sensitive sensor protein. Parallel work on EC funding has evaluated anti-auxin scFvs. We will introduce the anti-ABA scFv into various biosensor platforms: A sensor based on surface plasmon resonance and capable of multiple, parallel real-time monitoring of soluble analytes (patent application underway), conducting polymer based biosensors (with Prof Seamus Higson, Cranfield Biotechnology Centre) and a FRET cassette for expression in vivo (Drs David Vaux and Mark Fricker, Pathology and Plant Sciences, Oxford). Promising biosensors will be used for preliminary measurements of changing ABA and auxin concentrations. Mathematical descriptions of the data will be developed.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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