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509: Assessment of allelic diversity within Brassica

Reference	BBS/E/H/00GC0228
Principal Investigator / Supervisor	Dr Guy Barker
Co-Investigators / Co-Supervisors
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	237,489
Status	Completed
Type	Institute Project
Start date	01/04/2005
End date	31/03/2006
Duration	12 months

Abstract

Genetic analysis in Brassicas is underpinned by the use of segregating populations for which genetic linkage maps have been generated. The assignment and orientation of linkage groups in these maps is essentially arbitrary. There is now a pressing need to align these maps to standard reference maps. In addition, the analysis of genetic diversity Brassicas requires the use of molecular markers to link phenotypic variation to genetic linkage maps. We propose to address these two requirements in B. oleracea by: 1) Screening available parents of B. oleracea (C), B. rapa (A) and B. napus (AC) mapping populations with SSR markers and mapping these in the reference B. oleracea linkage map 2) Use mapped microsatellite markers to establish the molecular diversity within the B. oleracea Diversity Foundation Set being developed at WHRI. Microsatellite markers have properties that make them particularly valuable tools for comparative mapping as they are amenable to high throughput analysis, exhibit a high degree of polymorphism and are sequence tagged. Currently the number of microsatellite markers mapped in B. oleracea is limiting. By mapping microsatellite markers that have been mapped in other linkage maps in the B. oleracea reference map we will make valuable progress in being able to align these maps. The microsatellite marker-based diversity analysis of the B. oleracea Diversity Foundation Set will generate information on the genetic structure of the diversity in the set and provide the core data to which phenotypic analyses will be compared.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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