Award details

403e: Genome organisation and comparative functional genomics of Brassica oleracea

Reference	BBS/E/H/00032646
Principal Investigator / Supervisor	Dr Guy Barker
Co-Investigators / Co-Supervisors
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	382,400
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	31/03/2005
Duration	24 months

Abstract

Quality of horticultural produce is determined primarily through regulation of gene expression during plant development and in response to environmental variation. Key regulators of transcription orchestrate these processes by integrating interactions between genotype and environment. The Brassica oleracea (C) genome has arisen from recurrent segmental chromosome duplications. The contrast in organisation between duplicated regions provides an excellent experimental system to study mechanisms of gene regulation, as well as insights into the processes underlying chromosome duplication during polyploid evolution. We have established that around 70 per cent of the O6 linkage group is comprised of an inverted segmental duplication on the long chromosome arm, collinear with 11Mb of Arabidopsis Chr. I. Each segment contains paralogous genes including BoAP1-a and BoAP1-c, with the junction proximal to the self-incompatibility (S-) locus. Our objectives are a) Determine genome organisation of duplicated chromosomal segments; b) Understand mechanisms and consequences of divergence following segmental duplication; c) Develop an international B. oleracea EST programme to facilitate functional genomics and annotation. This will involve obtaining sequence information from around BoAP1-a and BoAP1-c and other regulatory genes; the comparison of allelic variation within the Brassica genepool; and the production and sequencing of stage specific B.oleracea EST libraries.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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