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403d: Functional analyses of novel genes controlling flowering in respect to photoperiod

Reference	BBS/E/H/00032644
Principal Investigator / Supervisor	Dr Stephen Jackson
Co-Investigators / Co-Supervisors	Professor Brian Thomas
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	551,500
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	31/03/2005
Duration	24 months

Abstract

The aim of this programme is to define a regulatory network of genes controlling the photoperiodic induction of flowering, which can be used to predict and/or manipulate flowering time. It will focus on four novel flowering time mutants that have been isolated in our group. All four mutants act specifically in the photoperiodic flowering pathway, goliath and O.3 are the best characterised of these mutants so far. Both GOLIATH and O.3 proteins contain a RING finger domain and thus are probably acting as E3 ligases controlling the ubiquitination of key regulatory proteins in the flowering pathway, in the case of O.3 we have preliminary evidence that it might affect the stability of CONSTANS. We intend to express these two proteins with an affinity tag in transgenic plants and perform pull-down experiments to isolate their protein partners. Yeast two-hybrid screening will also be used as a parallel approach. Transgenic plants (RNAi or SALK lines) where the expression of these proteins has been down-regulated will be assessed for the effect on flowering

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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