Award details

403b: Biosensors for plant hormones

Reference	BBS/E/H/00032640
Principal Investigator / Supervisor	Professor Richard Napier
Co-Investigators / Co-Supervisors
Institution	University of Warwick
Department	Warwick HRI
Funding type	Research
Value (£)	321,400
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	31/03/2005
Duration	24 months

Abstract

The proposal is directed at tissue-level, real-time biosensors for plant hormones ABA and auxin. Biosensors can be divided into reporter systems (biochemistry) and hardware (electronics). HRI has enthusiastic collaborators for the latter and the work in this project will be directed at identification and presentation of proteins suitable as reporters. The HRI group has established ABP1 as an ideal candidate sensor protein for an auxin biomonitor. The well- studied anti-ABA antibody fragment (scFv; Conrad and Weiler, Bochum) has been used by the research community for ABA immunoassays and when expressed in planta confers an ABA deficient phenotype, making it a candidate ABA sensor. HRIs Arabidopsis protein library display on virus lambda coupled with novel conjugates offers an additional, unique methodology for identifying receptor/sensor protein candidates. Additional technological advances are anticipated from the BBSRC Exploiting Genomics project running alongside this programmes. Objectives: (1)To exploit HRIs protein displays to discover novel hormone signalling elements (2)To assess proteins for suitability as durable biosensors (3)To design, make and test prototype sensors (collaborative, Cranfield, Warwick)

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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