Award details

Physical and genetic mapping of introgression and recombination of important traits in cereals

Reference	BBS/E/G/00003118
Principal Investigator / Supervisor	Dr Catherine Howarth
Co-Investigators / Co-Supervisors
Institution	Inst of Grassland and Environmental Res
Department	Inst of Grassland and Environmental Res Department
Funding type	Research
Value (£)	579,014
Status	Completed
Type	Institute Project
Start date	01/04/1999
End date	31/03/2003
Duration	48 months

Abstract

This project will use the combination of DNA markers, trait analysis and Fluorescence In Situ Hybridisation to help elucidate the underlying genetic and physiological basis of important traits and their introgression from wild to cultivated forms of cereals. A two-pronged approach to address these fundamental issues will be implemented: 1. Genetic mapping Resource allocation and end-use quality traits of the grain and the response to environmental conditions are the main target traits. Using a cross between two lines that contrast in seed protein and with-glucan content and in the naked grain character, an outline map of diploid oats will be produced using random genomic probes available from oats and other species. This will enable the alignment of this map with those produced elsewhere in oats and also with other gramineae in order to exploit potential syntenic relationships. The development and use of precise screening techniques for the traits of interest will also be required along with characterization of appropriate germplasm. Once markers linked to these traits are identified we can use them to follow the introgression of desirable QTLs into the hexaploid whilst also monitoring the introgression of undesirable traits. It will be possible using RFLP markers of known map location and positive BAC clones identified using these markers to assign linkage groups to chromosomes using FISH. Objectives are to: (a) Generate F2 A. atlantica x A. strygosa mapping families along with parental genotypes and regenerate the F1 A. atlantica (Cc7277) x A. strygosa cross; (b) To produce an outline genetic linkage map of diploid oats and to screen for traits of interest; (c) To investigate the effect of chromosome doubling on beta-glucan content and grain size; (d) Evaluate selected wild species, ecotypes and cultivated oats from within the oat collection to evaluate for drought resistance and nutrient use efficiency. 2. Physical location of sites of integration The introgression of alien DNA and chromosome rearrangements will be studied by physically mapping rearrangements and transgenic inserts via FISH. Utilising a number of lines, the position of insertion(s) and rearrangements within the genomes of the cultivated oat will be established. The chromosomal location of the respective transgenes will be assigned by producing hybrids between the transgenic lines and the monosomic series. This will enable us to test the hypothesis that there are sites of preferential integration hot spots within the genome and ascertain if the known C genome chromosome rearrangements or other integrations occur more frequently in these chromosome regions. The inheritance and expression of the transgenes in the hybrids produced from the monosomic analyses involving different transgenic events (and novel transgenic lines being produced at present involving different parental backgrounds) will be analysed with regard to positional integration and parental origin. Some integrations are known to involve more than one chromosome and multiple inserts in the same chromosomal region have been observed. The expression and inheritance such integrations will form an integral part of the project. Objectives are to: (a) pursue different techniques to identify transgenic insert into cultivated oat and establish transmission of transgenes; (b) Attempt to physically map the position of transgenic inserts via FISH, utilising independent co-transformation events and other co-integration events; (c) Endeavour to establish the precise chromosomal location of the respective transgenes by producing hybrids between the transgenic lines and the monosomic series; (d) Analyse the inheritance and expression of the transgenes in the hybrids produced from the monosomic analyses involving different transgenic events (and novel transgenic lines being produced at present involving different parental backgrounds).

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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