Award details

Rational design of novel fungicides effective against plant pathogens

ReferenceBBS/E/F/04330966
Principal Investigator / Supervisor Dr Anthony Michael
Co-Investigators /
Co-Supervisors
Institution Quadram Institute Bioscience
DepartmentQuadram Institute Bioscience Department
Funding typeResearch
Value (£) 10,930
StatusCompleted
TypeInstitute Project
Start date 01/04/1997
End date 31/10/1999
Duration31 months

Abstract

This work aims to use a molecular biological approach to the rational design of new fungicides based on inhibition of fungal polyamine biosynthesis. Polyamine biosynthesis genes will be cloned from plant pathogenic fungi and their host plant. The cloning of the fungal biosynthetic ornithine and S-adenosylmethionine decarboxylase genes will be achieved by using degenerate oligonucleotides with the polymerase chain reaction. In plants, there is an additional biosynthetic gene, arginine decarboxylase, which will also be isolated using the same technique. These genes will be used to manipulate polyamine metabolism in the fungi as the basis for the development of a "smart" screen. Genes will also be expressed in E. coli so that purified proteins can be studied at the biochemical or structural level.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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