Award details

Structure determination and redesign of enzymes active in the synthesis and hydrolysis of alpha-glucans for creation of novel specificities - AGADE

Reference	BBS/E/F/04148172
Principal Investigator / Supervisor	Professor Gary Williamson
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	84,857
Status	Completed
Type	Institute Project
Start date	01/09/1998
End date	30/09/2000
Duration	25 months

Abstract

This is an EU funded project. The goals in AGADE (Alpha- Glucan Active Designer Enzymes) are specificity engineering of enzymes active on biotechnologically important a-glucans; dextrans; starch; and related oligo- and polysaccharides. This includes expansion and exploitation of insight in structure-specificity relationships of the target enzymes i) glucosyltransferases synthesising, from sucrose, a-glucans of different bond-type, length, and branching; ii) barley limit dextrinase acting on the a-1,6 branch points in starch and maltooligosaccharides; iii) cyclodextrinase hydrolysing cyclodextrins and showing dual (i.e. a-1,4 and a-1,6) bond- type specificity on non-cyclic substrates; and iv) mutants and fusions of barley a-amylase and the starch binding domain of A. niger glucoamylase. This project contributes to the expertise in molecular biology and enzymology of glucosidases which are important for health benefits of phytochemicals

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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