Award details

Spatial localisation of proliferating and apoplotic cells in colon crypts

Reference	BBS/E/F/02300916
Principal Investigator / Supervisor	Dr Ian Johnson
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	8,794
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	17/07/1997
Duration	3 months

Abstract

The human colonic mucosa is composed of an array of crypts - blind ending glandular structures - which are the basic units of cell proliferation. Crypt cell proliferation is mediated by a variety of external signals derived from the extracellular matrix and the gut lumen including growth factors, cytokines, hormones and neurotransmitters, many of which are stimulated, directly or indirectly by diet. In order to define the effects of diet more precisely in health and disease, it is important to understand the intracellular messengers which transduce the signals reaching the crypt from the gut lumen and the surrounding tissues. Previous studies have shown that a number of secretagogues mobilise intracellular calcium, which is known to couple signal to responses such as proliferation and secretion in other tissues, but this mechanisms has never been studied in intact crypts which retain their spatial and functional integrity. The aim of this project is to develop an isolated tissue preparation which will enable the effects on calcium signalling of conventional secretagogues, and constituents of the colonic lumen such as plant secondary metabolites, fermentation products and minerals, to be studied in isolated intact crypts derived from human tissue.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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