Award details

Purification and characterisation of esterase capable of degrading plant cell walls

Reference	BBS/E/F/02150910
Principal Investigator / Supervisor	Dr Paul Kroon
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	4,355
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/06/1997
Duration	3 months

Abstract

Previous work on cell wall-degrading esterases has led to the isolation and characterisation of several enzymes, the most studied being FAE-III and CinnAE from Aspergillus niger. Of all the enzymes so far purified however, none shows a preference for one of the feruloyl-sugar linkages found in certain plant cell walls (a-1,6-linkages to galactose). FAE-III shows highest activity on 1,5-links to arabinose, whereas CinnAE is most efficient on 1,2-links to arabinose. We have recently detected an enzyme which is active on esters of sinapic acid (hence the name, sinapic acid esterase, SAE) and preliminary experiments show that it has a preference for 1,6 linked feruloylated galactose oligosaccharides derived from cell walls. We intend to purify SAE, and to determine its precise substrate for cell wall structures.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search