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Characterisation of human transcription factors that mediate gene expression via antioxidant responsive element

Reference	BBS/E/F/02150852
Principal Investigator / Supervisor	Professor Gary Williamson
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	87,261
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/1999
Duration	24 months

Abstract

In the course of our work on the protective effects of fruit and vegetables against chronic disease, we demonstrated the existence of a novel transcription factor in the nuclei of human cells. We think that this protein is the molecular signal that binds to the antioxidant responsive element, and controls expression of many genes in response to dietary nutrients and non-nutrients. In this project, we propose to exploit this finding by cloning and purification of this transcription factor. We will obtain enough protein to perform structure-function studies and produce antibodies to enable quantitation of the levels in human tissues, and we will express the protein in a human cell line to elucidate its exact intracellular function. The results will provide a central rationale for some of the anticancer effects of fruit and vegetables at a molecular level. This multidisciplinary project combines protein chemistry, molecular biology, genetics and biochemistry with a nutrition-related objective. Work in this area is supported by a related EU-FAIR project and a MAFF Open Contract, and nutrients and gene expression is a priority area within BBSRC.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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