Award details

Preparation & characterisation of multiply branched glucose oligosaccharides for structural investigations of amylopectin & glycogen

Reference	BBS/E/F/02100839
Principal Investigator / Supervisor	Dr Ian Colquhoun
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	24,790
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/05/1999
Duration	26 months

Abstract

The aim is to develop a protocol which will provide information on the distribution of branched glucose residues in amylopectin and glycogen. The physical and technological properties of modified starches, e.g. from mutant pea varieties, are being actively studied. Although differences in amylopectin chain profiles have been determined, little is known about variations in branch point distributions and their relationship to functional properties. Progress towards tailoring amylopectin structures through plant genetic engineering emphasises the need for new methods of structural analysis. This project will exploit particularly the modern techniques of high performance anion exchange chromatography and two-dimensional NMR spectroscopy to characterise the mixtures of oligosaccharides produced by a- -amylase degradation of the polysaccharides. Clustering of branch points in the polysaccharide results in production of multiply branched a--dextrins in which the arrangement of closely spaced branch points is preserved. Modifications of the polysaccharide structure will be evident from the profiles of degradation products obtained. The purified oligosaccharides will also be used as model substrates in planned studies of novel debranching enzymes.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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