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Developing new biomarkers of colorectal noeplasia: Quantification of aberrant CpG island methylation in human faecal DNA

Reference	BBS/E/F/00051588
Principal Investigator / Supervisor	Dr Ian Johnson
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	4,887
Status	Completed
Type	Institute Project
Start date	01/10/2002
End date	30/11/2005
Duration	38 months

Abstract

Colorectal cancer develops from adenomatous polyps that emerge gradually from aberrant fields of mucosal epithelial cells. Biomarkers are needed for research into the role of diet in the early stages of this common condition. We have developed 2 new methods to detect (Methylation-Specific PCR), and quantify (Methylation-specific restriction fragment length polymorphism) CpG island hyper-methylation in human faecal DNA, derived from colorectal epithelial cells. We now propose to use faecal samples to determine the occurrence of abnormal CpG island methylation, at 5 target genes, in both healthy volunteers and patients. We will also use a PCR-augmented genomic DNA subtraction technique to identify novel genes acquiring CpG island methylation early in the onset of neoplasia. Finally we will search for patterns of hyper-methylated genes that define early stages of the adenoma-carcinoma sequence, and design protocols for their use as faecal biomarkers in epidemiological and dietary studies.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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