Award details

Linking genotype and transcriptome response variation of Salmonella Typhimurium to pathogenicity and survival during transmission from farm to fork

Reference	BBS/E/F/00044408
Principal Investigator / Supervisor	Professor Robert Kingsley
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	1,032,374
Status	Current
Type	Institute Project
Start date	01/01/2014
End date	31/03/2018
Duration	50 months

Abstract

Using a diverse collection of field isolates of Salmonella Typhimurium we will identify genotypic features associated with variants that successfully enter the food chain and cause disease in man. Distinct genotypes exhibit different likelihood of entering the food chain and causing disease. For example two genotypic variants commonly isolated from pigs in the last 10 years in the UK, but only one is commonly associated with disease in man. We will address the hypothesis that lineage specific genetic factors impacting survival in the food chain, or the transcriptional response to environmental signals account for the absence of common livestock genotypes entering and causing disease in man. We will determine in representative isolates from across the tree, the diversity of genotype and coding capacity, and transcriptional response to stress factors encountered within the food chain such as low pH, heat, osmotic stress, desiccation and survival / replication in nutrient poor environments. We will investigate the diversity of the S. Typhimurium interaction with the intestinal mucosa. We will address the hypothesis that while some S. Typhimurium variants may enter the food chain they are not a common cause of human disease due to genotype-specific coding capacity or transcriptome response that modulates interactions with the host. We will employ saturating transposon mutagenesis with TraDIS to define essential genes in distinct Typhimurium lineages for survival in the inflamed intestine and survival in stress conditions, an important factor in the transmission of these isolates during infection.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Microbial Food Safety, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search