Award details

Metabolomics

Reference	BBS/E/F/00042295
Principal Investigator / Supervisor	Dr Ian Colquhoun
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	736,376
Status	Completed
Type	Institute Project
Start date	01/04/2005
End date	01/04/2009
Duration	48 months

Abstract

Metabolomics is entering a new era at IFR with the installation of an LC/SPE/NMR/MS instrument. In conjunction with existing GC/MS, LC/MS/MS and HPLC/UV instruments, metabolomics at IFR will become a highly sophisticated science with a more coherent approach to the integrated use of these complementary technologies than was possible before. In particular, the analysis of complex mixtures will be carried out with greater resolution, sensitivity and throughput. By capturing all the relevant spectroscopic information at once and then mining the data with multivariate methods we will be able to quantify multiple components and locate and identify novel compounds much more rapidly. Two keys to this are the NMR cryoprobe for high sensitivity LC/SPE/NMR and the time of flight mass spectrometer, which gives immediate access to reliable empirical formulae for unknown compounds. Metabolite profiling of plants and foods and metabolite identification are areas in which IFR has an international reputation. We aim to build on this expertise and also to develop metabolomic analyses of biofluids, tissues and bacterial extracts that will be of equal benefit to the nutrition, health and microbial research at the Institute. In more fundamental studies we will contribute to functional genomics and systems biology developments through metabolomics, transcriptomics and proteomics experiments carried out in parallel. Because of its high sample throughput, metabolomics will often be used initially to select the most interesting candidates for measurement by the other two techniques.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Diet and Health, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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