Award details

Molecular microbiology of Salmonella

ReferenceBBS/E/F/00042252
Principal Investigator / Supervisor Dr Arthur Thompson
Co-Investigators /
Co-Supervisors
Professor Jay Hinton
Institution Quadram Institute Bioscience
DepartmentQuadram Institute Bioscience Department
Funding typeResearch
Value (£) 2,129,333
StatusCompleted
TypeInstitute Project
Start date 01/04/2005
End date 31/03/2010
Duration60 months

Abstract

Salmonella is a leading cause of food-borne bacterial infections which are responsible for significant problems in the UK. Because transmission and infection are dependent on the survival of bacteria in different environments such as water, soil and food, we are investigating the effect of environmental change on the expression of genes involved in infection. We are capitalising on the complete DNA genome sequences now available for Salmonella. Our functional genomic strategy involves DNA microarray-based approaches to study bacterial genes that are switched on or off by environmental signals, or during the infection process. We are also investigating how Salmonella genes are switched on during infection or within bacterial populations by monitoring gene expression in individual Salmonella cells. This involves the use of Green Fluorescent Protein (GFP) as a reporter to give a visual indication of the switching on of particular genes during infection of mammalian cells, which is observed by microscopy or measured by flow cytometry. The understanding of pathogenesis and environmental stress responses that we are developing for Salmonella will open up new possibilities for the control or eradication of harmful bacteria from the food chain.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsAnimal Health, Microbial Food Safety, Microbiology
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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