Award details

Molecular microbiology of Salmonella

Reference	BBS/E/F/00042252
Principal Investigator / Supervisor	Dr Arthur Thompson
Co-Investigators / Co-Supervisors	Professor Jay Hinton
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	2,129,333
Status	Completed
Type	Institute Project
Start date	01/04/2005
End date	31/03/2010
Duration	60 months

Abstract

Salmonella is a leading cause of food-borne bacterial infections which are responsible for significant problems in the UK. Because transmission and infection are dependent on the survival of bacteria in different environments such as water, soil and food, we are investigating the effect of environmental change on the expression of genes involved in infection. We are capitalising on the complete DNA genome sequences now available for Salmonella. Our functional genomic strategy involves DNA microarray-based approaches to study bacterial genes that are switched on or off by environmental signals, or during the infection process. We are also investigating how Salmonella genes are switched on during infection or within bacterial populations by monitoring gene expression in individual Salmonella cells. This involves the use of Green Fluorescent Protein (GFP) as a reporter to give a visual indication of the switching on of particular genes during infection of mammalian cells, which is observed by microscopy or measured by flow cytometry. The understanding of pathogenesis and environmental stress responses that we are developing for Salmonella will open up new possibilities for the control or eradication of harmful bacteria from the food chain.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Animal Health, Microbial Food Safety, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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