Award details

IIA Studentship: Selenium and Prostrate Cancer Prevention

Reference	BBS/E/F/00042180
Principal Investigator / Supervisor	Dr Francis Mulholland
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	51,600
Status	Completed
Type	Institute Project
Start date	01/10/2008
End date	30/09/2011
Duration	36 months

Abstract

The hypothesis that will be tested in this studentship is that selenium can modulate levels of various types of collagen to inhibit the reactive stroma effect and prevent prostate cancer growth/progression/invasion. The student will undertake mechanistic studies using cultured prostate cells to investigate effects of different forms and doses of selenium on several key types of collagen in relation to prostate cancer growth, and to study the relationship between collagen formation and breakdown. Confocal microscopy imaging techniques will be used to visualise the types of collagen and study structural changes in collagen and the extracellular matrix, and the relationship between selenium status, key gene/protein expression and tissue phenotype will be investigated. Project Outline: Investigate mechanisms of anti-cancer effects of selenium and biomarkers of selenium status in prostate cells/tissue. Determine effect of dose and form of selenium on global protein expression in prostate cells using proteomics techniques. Optimise methods (protein extraction and electrophoresis conditions?) to include analysis of extracellular matrix proteins? Comparison between effects of Selenomethionine and Se-methylselenocysteine and effects of multiple forms combined Use of human prostate cells normal/cancer model Identify Selenium-responsive proteins using MALDI-TOF or other suitable mass spec identification techniques, and confirm change using western blotting/enzyme activity and /or ELISA approach Explore possibility of using prostate tissue explants and 3D cell culture model to identify mechanistic effects of selenium on prostate tissue - proteomics approach? Investigate the effects of different forms and doses on selenium on the plasma proteome using human samples from selenium dietary intervention study - (identify Se-responsive proteins and mechanisms of effect) Study cellular effects of selenium on collagen production and extracellular matrix using confocal microscopy techniques

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Diet and Health
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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