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Award details
Functional genomics and metabolics of the versatile Acyl-CoA Transferase super-family of enzymes in plants
Reference
BBS/E/F/00041880
Principal Investigator / Supervisor
Dr Anthony Michael
Co-Investigators /
Co-Supervisors
Institution
Quadram Institute Bioscience
Department
Quadram Institute Bioscience Department
Funding type
Research
Value (£)
123,898
Status
Completed
Type
Institute Project
Start date
01/02/2005
End date
31/01/2008
Duration
36 months
Abstract
The objective of this proposal is to take a systematic approach to identifying the function of versatile acyl transferase (VAT) family members from Arabidopsis to define new activities. We aim to identify the biochemical function of ten selected members of the AtVAT family. We will do this using a systematic functional genomics/metabolomics approach. We will isolate lines mutant for each VAT activity and characterise the changes in plant secondary metabolism as a result of loss of each activity. Such 'metabolic phenotypes' will be defined by state-of-the-art GC/MS. LC/MS and LC/MS/MS technology. Establishing which metabolites have altered will allow us to deduce the reaction catalysed by each VAT. Activities will be confirmed by in vitro assays. We will also test whether modulation of the activity of VATs in plants gives rise to changes in natural product accumulation, and whether such traits offer increased tolerance to stresses, that may be useful traits for crop plants.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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