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Health benefits of broccoli
Reference
BBS/E/F/00041700
Principal Investigator / Supervisor
Professor Richard Mithen
Co-Investigators /
Co-Supervisors
Institution
Quadram Institute Bioscience
Department
Quadram Institute Bioscience Department
Funding type
Research
Value (£)
44,920
Status
Completed
Type
Institute Project
Start date
01/04/2003
End date
31/03/2012
Duration
108 months
Abstract
The project seeks to explore changes in human gene expression in vivo following consumption of standard broccoli and isothiocyanate-enhanced broccoli. Two main human intervention studies will be undertaken in collaboration with the Division of Gastroenterology, University of Nottingham. The first will involve a cross over study with volunteers fed broccoli, ITC-enhanced broccoli or a control meal. Gastric biopsies will be obtained, and gene expression quantified through the use of GeneChip technology and rt-pcr. The second study will be similar in design, but will involve obtaining colonic biopsies following broccoli consumption. A third human intervention study will be undertaken at IFR, in which the metabolism of isothiocyanates and other broccoli secondary metabolites will be quantified. Changes in gene expression in lymphocytes will also be investigated. Studies on the effect of broccoli extracts on gene expression in cell cultures will be undertaken in parallel to the human intervention studies. In addition, the project involves characterisation of the secondary metabolites of standard and ITC-enhanced broccoli when grown in different sites, in the UK, Holland and USA. The project funds a PhD student associated costs.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
Crop Science, Diet and Health, Plant Science
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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