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Molecular investigation of mechanisms of copper homeostasis in humans
Reference
BBS/E/F/00041686
Principal Investigator / Supervisor
Professor Susan Jane Fairweather-Tait
Co-Investigators /
Co-Supervisors
Institution
Quadram Institute Bioscience
Department
Quadram Institute Bioscience Department
Funding type
Research
Value (£)
71,065
Status
Completed
Type
Institute Project
Start date
01/01/2004
End date
31/03/2007
Duration
39 months
Abstract
This aim of this project is to investigate the genetic regulation of copper homeostasis using a dose and time-dependent approach. The response of the principal sites of absorption and excretion (i.e. the GI tract and the liver) will be investigated with human cell lines. In parallel, a lymphocyte cell line will be investigated to determine whether these reflect changes in transcription and/or translation as this is the most accessible target tissue for future human studies. Initially, changes in expression of key genes associated with copper homeostasis will be investigated using real-time RT-PCR (Taqman) in order to identify critical time points and doses. Subsequently, global levels of transcription and translation will be determined using the IFR human microarray and proteomic techniques, under conditions selected from the results of the preliminary experiments. This should simplify the hunt for common profiles of expression in clusters of genes linked to specific aspects of copper metabolism and trafficking, and may indicate suitable biomarker(s) of copper status. The large data sets generated will be analysed using appropriate software, in collaboration with the Bioinformatics team at IFR.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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