Award details

Development of DNA microarray for nutritional studies

Reference	BBS/E/F/00041371
Principal Investigator / Supervisor	Dr Ruan Elliott
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	804,882
Status	Completed
Type	Institute Project
Start date	01/04/2000
End date	31/03/2004
Duration	48 months

Abstract

The objective of this project is for IFR to produce its own high quality human DNA microarrays for use in studies of the interactions between dietary components, gene expression and cellular function. The arrays will be produced using robotic systems already in place. The human arrays are based on synthetic oligonucleotides (each 70 nucleotides long). A set of 14,000 of these oligonucleotides, derived from the human UniGene database, has been purchased. Each oligonucleotide has been designed to measure the expression of a specific, well-characterised human gene. The sequences of all the oligonucleotides have been optimised to (i) match their annealing temperatures so as to obtain consistent stringency across the array, (ii) minimise secondary structures and (iii) maximise specificity, thereby reducing cross-hybridization and non-specific background. This basic oligonucleotide set provides coverage of between one half and one third of all human genes. Individual oligonucleotides will be added to the set to ensure representation of all identified genes of particular interest to given projects. In addition, commercial "add-on" sets of oligonucleotides will be purchased as they become available. In this way, the arrays will constantly adapt to our specific requirements and expand with our increasing understanding of human genes.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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