Award details

Molecular microbiology of salmonella typhimurium and E. Coli

Reference	BBS/E/F/00041208
Principal Investigator / Supervisor	Professor Jay Hinton
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	2,746,596
Status	Completed
Type	Institute Project
Start date	01/04/2000
End date	31/03/2005
Duration	60 months

Abstract

Salmonella and E. coli are leading causes of food-borne bacterial infections which are causing significant problems in the UK. Because transmission and infection are dependent on the survival of bacteria in different environments such as water, soil and food, we are investigating the effect of environmental change on the expression of genes involved in infection. We are capitalising on the complete DNA genome sequence now available for E. coli, and will use the Salmonella genome sequence which is due in late 2000. Our functional genomic approach will use DNA microarrays and proteomic approaches to study bacterial genes that are switched on or off by environmental signals, or during the infection process. We are also investigating how Salmonella genes are switched on during infection by monitoring gene expression in individual Salmonella cells. This involves the use of Green Fluorescent Protein (GFP) as a reporter to give a visual indication of the switching on of particular genes during infection of mice, which is observed by microscopy or measured by flow cytometry. Our understanding of pathogenesis and environmental stress responses will open up new possibilities for the control or eradication of harmful bacteria in the food chain

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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