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The Role of CD45 Isoforms in Lymphocyte Signalling

Reference	BBS/E/E/0CD/1/00
Principal Investigator / Supervisor	Professor Peter Beverley
Co-Investigators / Co-Supervisors	Dr D Alexander
Institution	Edward Jenner Institute for Vaccine Res
Department	Scientific Head
Funding type	Research
Value (£)	50,000
Status	Completed
Type	Institute Project
Start date	11/01/2001
End date	10/01/2004
Duration	36 months

Abstract

CD45 is a major surface molecule in all leucocytes and is a tyrosine phosphatase. This project will investigate the role of individual CD45 isoforms in regulating signals transduced by the T cell receptor and by the CD28 and CTLA-4 co- receptors. T cells from single isoform transgenic mice bred on to the knockout background will be used. The immunological phenotype and function of these mice will be assessed using immunofluorescence and flow cytometry, immunohistology, in vitro mitogen assays and immunisation with specific antigens. These data will be correlated with the results of signalling assays. Kinase regulation in thymocytes and peripheral T cells will be assayed by immunoblotting of precipitated kinases to assess phosphorylation status, in vitro assays of kinase immunoprecipitates, and studies of kinase TCR interactions. Interactions between CD45 isoforms and other cell surface molecules will be studied using Fluorescence Energy Resonance Transfer (FRET).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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