Award details

Development and exploitation of animal biotechnologies

Reference	BBS/E/D/20221658
Principal Investigator / Supervisor	Professor Helen Sang
Co-Investigators / Co-Supervisors
Institution	University of Edinburgh
Department	The Roslin Institute
Funding type	Research
Value (£)	2,657,340
Status	Completed
Type	Institute Project
Start date	01/04/2012
End date	31/03/2017
Duration	59 months

Abstract

Genetic modification in the mouse has provided increasingly sophisticated tools for investigating all aspects of development and physiology of this model vertebrate. The Roslin Institute has a strong track record in development and application of genetic modification technologies in farm animal species. These have provided powerful tools to answer fundamental biological questions while at the same time providing the platform for the development of novel commercial opportunities. This theme aims to enhance animal transgenesis through novel stem cell based approaches and direct embryo manipulation methodologies. We will exploit novel strategies for animal stem cell derivation, based on the induction of pluripotent stem cells (iPS cells) by expression of key transcription factors in differentiated cells, as exemplified by Takahashi and Yamanaka. We will develop robust culture protocols for such cells and use them as tools for genetic modification and develop differentiation protocols, for applications in animal production and basic bioscience underpinning animal and human health. In parallel, for poultry, we have established and advanced the culture of chicken primordial germ cells, the precursors of the gametes, which will enable us to develop cell-based methods for genetic modification of poultry. We will develop more sophisticated genetic modification technologies, using these novel cell-based systems and by direct in vivo manipulation, including gene knockout and gene targeting in pigs, sheep, cattle and poultry. These technologies will be applied in a wide range of programmes, in collaborative projects, jointly with other ISPs, external collaborators and industry.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Animal Health, Stem Cells
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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