Award details

Isolation and characterization of fungal genes involved in biotrophy & plant-fungal recognition in the Colletotrichum lindemithianum-bean interaction

Reference	BBS/E/C/00823797
Principal Investigator / Supervisor	Professor Gerald Bennett
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	88,392
Status	Completed
Type	Institute Project
Start date	01/10/1997
End date	30/09/2000
Duration	36 months

Abstract

Details should only be considered in the context of PU information. This project, involving close collaboration with University of Birmingham aims to identify and characterise genes expressed during biotrophic interactions between Colletotrichum lindemuthianum and bean (Phaseolus vulgaris). This hemibiotroph is used as a model system because, unlike obligate biotrophs, it can be cultured and transformed, allowing critical assessment of gene function. Using monoclonal antibodies (MAbs) we have identified two biotrophy- related proteins and a full length cDNA corresponding to one of these was obtained by immunoscreening an expression library prepared with RNA from infected tissue. It codes for a fungal proline-rich protein (CIH1) that forms a multimeric structure at the plant-fungal interface. A second protein (CIH2) in the cytoplasm of biotrophic hyphae appears to be race-specific. We aim to (a)examine the role of both proteins in pathogenicity, biotrophy and race- specificity, (b)search for homolgues in other biotrophic fungi, (c)study the mechanism by which the proline-rich protein forms a multimeric structure and (d)determine the effects on pathogenicity of injecting antibody fragments into infected tissue.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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