Award details

Recognition domains and signal transduction in plant:pathogen specificity proteins

Reference	BBS/E/C/00823354
Principal Investigator / Supervisor	Professor John Lucas
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	124,787
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/1999
Duration	24 months

Abstract

Specific recognition of plant pathogens is often determined by single dominant recognition (R) genes encoding proteins postulated to act as receptors for pathogen elicitor molecules. The sequences of several R genes and their products are now known but functional details, including the recognition mechanism and subsequent signal transduction, are not clear. This project is analysing functional aspects of the recognition process by domain engineering and expression in a model assay system. Objectives 1996 To confirm function of N gene in tobacco and esetablish a transient assay for gene expression. To define recognition domain by deletions and expression in transgenic tobacco. To epitope tag N protein in constructs to allow localization in planta. 1997 The N gene conferring resistance to tobacco mosaic virus was selected for this project as it is believed to have advantages for use in functionality assays. The putative leucine rich repeat (LRR) domain from the gene has been cloned and expressed in E. coli. Initial attempts to identify the specific elicitor from TMV using precipitation assays were unsuccessful. The focus of the work now is to alter the protein binding specficity of the LRR by mutation and test in transgenic tobacco.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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