Award details

Isolation & characterisation of pathogenicity genes from wheat pathogen Pseudocercosporella herpotrichoides

Reference	BBS/E/C/00823333
Principal Investigator / Supervisor	Dr Paul Bowyer
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	96,969
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/1999
Duration	24 months

Abstract

Pseudocercosporella herpotrichoides is one of the most damaging fungal pathogens of wheat. Though the physiology of infection, sexual crossing and race structure have all been extensively studied in this plant pathogenic fungus little atttention has been paid to the genes and proteins responsible for these processes. We propose to dissect the genetic mechanisms of pathogenicity using novel insertional mutagenesis techniques such as REMI and signature tagged REMI which potentially allow simultaneous identification of pathogenicity genes by negative selection. Genes identified by this procedure will be sequenced and compared with existing databases to gain insight into their function. Particular attention will be given to genes in which mutation prevents development of infection structures such as appressoria, infection plaques and runner hyphae. Differential cDNA screening procedures will be used to isolate genes expressed specifically in various stages of disease development. The genes required for pathogenicity in this fungus will be manipulated by over-expression to form the basis of smart screens to discover improved forms of fungicide effective against P. herpotrichoides. Objectives 1996 The first set of objectives for this project are to establish a range of molecular techniques or tools for use in P. herpotrichoides. Initially this will entail: establishment and optimisation of transformation systems, construction of vetcors for transformation and REMI, establishment of REMI technology and construction of signature tag libraries in REMI vectors. cDNA and genomic libraries will be constructed and infection processes studied as the basis for isolation of stage specific cDNA clones. 1997 Not supplied.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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