Award details

Monoclonal antibodies for wheat end use quality.

Reference	BBS/E/C/00823257
Principal Investigator / Supervisor	Dr Arthur Tatham
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	26,328
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	01/04/2001
Duration	48 months

Abstract

Details should only be considered in the context of PU information. To develop monoclonal antibodies to wheat gluten proteins for use in test kits for plant breeders and grain processors. Antibodies are being raised against total gliadins, total glutenins, individual gliadins and glutenin subunits and synthetic peptides based on their amino acid sequences. The antibodies are being characterized by western blotting and ELISA assays and will be used to determine total gluten in wheats and specific proteins associated with different quality parameters, for example, for making bread, cakes and biscuits and novel end uses such as extrusion and texturing. The antibodies will also be used to determine the effects of climate and nutrition on gluten content and quality, and to examine patterns of deposition during development. The project is strongly collaborative with IFR, Norwich Laboratory, and industrial partners. It is currently supported by DTI under the Agrofood Quality LINK scheme, but forms part of a larger team project in the BBSRC Directorate Strategy area 'Raw Material Quality'.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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