Award details

Mechanisms of protein synthesis, processing, targeting and deposition in plant cells

Reference	BBS/E/C/00823251
Principal Investigator / Supervisor	Professor Johnathan Napier
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	90,581
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/1999
Duration	24 months

Abstract

Storage proteins are major determinants of the functional properties and end use properties of seeds, including the breadmaking quality of wheat. In order to understand these properties, and to exploit them in the food or other industries, it is necessary to understand how the proteins are synthesised, processed and trafficked in the cells of the developing seed. Work in this RO focuses on the basic biology of these processes, with emphasis on the gluten proteins of wheat. It includes studies of protein translocation into the lumen of the ER, folding and assembly in the ER lumen (including the roles of chaperones and PDI), trafficking through the secretory pathway (including the role of small GTP-binding proteins) and deposition into protein bodies. Objectives 1996 Continue studies of the role of small GTP- binding proteins in trafficking. Study the role of BiP and PDI in folding of gamma-gliadin in vivo and in vitro. 1997 Generate transgenic wheat expressing wildtype and trans- dominant forms of the tobacco Rab1 small GTP-binding protein under the control of a seed specific promoter. Examine developing grains from these lines for alteration in the deposition of protein bodies, including the composition by various different seed storage proteins (gliadins versus glutenins). Finish work on ectopic expression of the seed- specific protein oleosin.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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