Award details

Characterisation of key genes in ATP-binding cassette transporters

Reference	BBS/E/C/00014204
Principal Investigator / Supervisor	Dr Julian Coleman
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	200,673
Status	Completed
Type	Institute Project
Start date	01/04/1999
End date	31/03/2001
Duration	24 months

Abstract

Plants have versatile detoxification systems to counter the phytotoxicity of a wide variety of natural and synthetic chemicals (xenobiotics). An important cellular detoxification mechanism is the chemical modification of the xenobiotic by conjugation to endogenous metabolites such as glutathione or glucose, followed by the export of the conjugates from the cytosol to the vacuole or the apoplast. The aim of this project is to identify and characterise the key genes and proteins responsible for conjugation and transport in the cereals Hordeum vulgare (barley) and Triticum aestivum (wheat), and in herbicide-resistant populations of the grass weeds Alopercurus myosuroides (black-grass) and Avena spp. (wild oats). Special emphasis will be placed on ATP-binding cassette (ABC) transporters, in particular how their temporal and spatial expression affects the metabolism and fate of xenobiotics in planta. At the cellular level,conjugation and compartmentation mechanisms will be studied in cultured cells, protoplasts and isolated vacuoles using fluorescent and radioactive tracers. Expression analysis at gene and protein levels will be carried out by Northern blotting and immunolocalisation respectively.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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